Nuciferin is an aporphine alkaloid, the main component of Lotus

leaves (Folium Nelumbinis), used as a marker for standardizing

LOWLP-PT dry extract. The extraction and quantification method

using high-performance liquid chromatography (HPLC) for this dry

extract has been developed and validated. The analysis was performed

on an HPLC system using a Phenomenex C18 column (250 × 4.6 mm,

5 μm), with the mobile phase consisting of a mixture of acetonitrile:

water: triethylamine: glacial acetic acid (27.5: 70.1: 1.63: 0.75 v/v),

operated in isocratic mode at a flow rate of 1.2 mL/min, with a sample

injection volume of 10 μl and a detection wavelength of 284 nm using

a PDA detector. The method has been validated and complies with

ICH and AOAC guidelines. Using the developed method, the content

of nuciferin in three lots of the investigated formulation was

determined to be in the range of 0.171 - 0.194% based on the amount

of dried extract.