Diagnostic values of adenosine deaminase cytokines in fluid contributed to the detection of extrapulmonary tuberculosis
Huong Bui Thi Thu, Le-Thi Minh Hien, , , , , , , Nguyen Tien Dung, Sep-2024, In: Italian Journal of Medicine, 8, 3, p. 1810
Overview
Abstract:
Tuberculosis (TB) is a serious global public health problem.
Detecting the changes of adenosine deaminase (ADA),
interleukin (IL)-1β, IL-2, tumor necrosis factor-α (TNF-α),
interferon-γ (IFN-γ) released from macrophages and monocytes
is greatly valuable in the diagnosis of TB. Hence, this
study was designed to determine the optimal cut-off points of
ADA, IL-1β, IL-2, TNF-α, and IFN-γ, allowing to differentiate
pleural TB (PLTB), peritoneal TB (PTB), and meningeal
TB (MTB). The study included 386 patients with pleural effusion,
47 patients with peritoneal effusion, and 134 patients
with encephalitis and meningoencephalitis suspect of TB.
ADA was determined by an enzyme kinetics method; cytokine
concentration was measured by a test based on the
competitive enzyme-linked immunosorbent assay principle.
Sensitivity, specificity, and positive and negative predictive
value were calculated and described. In the aspiration fluid,
ADA activity was greatly different between PLTB, PTB, and
MTB groups with values of 37.5 U/L, 30.5 U/L, and 8.1 U/L
and sensitivity and specificity of 87.7% and 83.4%; 100% and
88.9%; 97.3% and 98.6%, respectively. Similar, IFN-γ concentrations
were distinctively different between PLTB, PTB,
and MTB patients (with values of 120 pg/mL, 200 pg/mL,
and 30 pg/mL with sensitivity and specificity of 80.3% and
80.9%; 80.0% and 92.6%; 98.6% and 61.7%, respectively).
The determination of ADA and IFN-γ levels in the aspiration
fluid is potentially considered a highly sensitive and specific
test for differentiating PLTB, PTB, and MTB.
Keyword(s): ADA, IL-1β, IL-2, TNF-α, IFN-γ, cytokines, tuberculosis pleura, peritoneum, meninges.
| Article number | 1810 |
| Journal | Italian Journal of Medicine |
| Volume | 8 |
| Issue number | 3 |
| Publication status | Published - Sep-2024 |
| ISBN | 1877-9344 |