Vancomycin, the first line antibiotic for methicillin-resistant Staphylococcus aureus (MRSA). However, an increasing number of MRSA isolates with high MICs, within the susceptible range (vancomycin MIC creep), are being reported worldwide. This study aims to determine the minimum inhibitory concentration (MIC) of vancomycin for MRSA at Thainguyen Central Hospital. Methods: Cross-sectional descriptive research. Collection of 140 MRSAs isolated infection at Thainguyen Central Hospital from 01/2020 to 12/2020. Determination of MRSA by using the Kirby-Bauer disk-diffusion technique. MIC of vancomycin to confirmed MRSA strains were determined by Etest method. Results: A total of 140 S. aureus isolates from different specimens in this study; MRSA rate is 61,43% (86 strains), Minimum inhibitory concentrations of vancomycin to the strains of MRSA ranged from 0.5 μg/ml to 2 μg/ml. The number of strains with MIC of 1.5 μg/ml -2 μg/ml were 44 (51,14%). Most of the MRSA strains were resistant against multiple classes of commonly used antibiotics.