Research objectives: To assess knowledge, attitude and practices (KAP) of parents of pediatric patients with thalassemia treated at Thai Nguyen National General Hospital in 2020.  Research methods: Description, the design of cross-sectional study. Research results: Rates of parents of pediatric patients demonstrated good and excellent levels of knowledge and attitude towards thalassemia prevention were 31,1% and 68.9% respectively. 20% of parents of pediatric patients voluntarily test for detection of thalassemia disease. The proportion of pediatric patients’ parents who ever counseled their relatives to test for detection of thalassemia disease was 36.7%; actively seeking information and sharing knowledge about thalassemia disease with others of parents of pediatric patients accounted for 74.4% and 65.0% respectively. Conclusion: Knowledge, attitude, and practices of parents of pediatric patients towards Thalassemia prevention are not yet positive.