Objectives: To evaluate the association between fetal ventricular septal defect (VSD) and chromosomal abnormalities. Subjects and methods: The 132 fetuses diagnosed VSD in the National Hospital of Obstetrics and Gynecology from January 2017 to March 2018 were included. Complicated with other cardiac abnormalities/extracardiac abnormalities or not, the cases were divided into isolated VSD group, VSD complicating cardiac anomalies group (other cardiac and/or great vessels malformation), VSD complicating extracardiac anomalies group (include organ malformation and sonographic soft markers) and VSD with both cardiac and extracardiac anomalies group. Results: Among the 132 VSD fetuses, 44/132 (33.3%)were isolated VSD; 31/132 (23,5%) were cases with other cardiac anomalies; 52/132 (39.4%) were cases with extracardiac anomalies and 5/132 (3.8%) were cases with both cardiac and extracardiac anomalies. The chromosomal karyotypes were obtained in 45 cases. Of all these 45 cases, 20/45 (44,4%) had chromosomal abnormalities, abnormalities encountered with trisomy 18, trisomy 21, trisomy 13. Conclusions: Fetal VSD had the highest risk of chromosomal abnormalities, especially the inlet type and VSD with extracardiac abnormalities, and then the fetal karyotype should be recommended.